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KMID : 0624620080410040287
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BMB Reports
2008 Volume.41 No. 4 p.287 ~ p.293
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BRI3 associates with SCG10 and attenuates NGF-induced neurite outgrowth in PC12 cells
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Gong Yanhua
Wu Jing
Qiang Hua
Liu Ben
Chi Zhikai
Chen Tao
Yin Bin
Peng Xiaozhong
Yuan Jiangang
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Abstract
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In a yeast two-hybrid screen, we identified the microtubule-destabilizing protein SCG10 as a potential effector protein of BR3. The association was verified using GST pull-down, Co-IP, and their perinuclear co-localization. The analysis of in vitro microtubule polymerization/depolymerization showed that the binding of BR3 to SCG10 effectively blocked the ability of SCG10 to induce microtubule disassembly, as determined by turbidimetric assays. In intact PC12 cells, BR3 exhibited the ability to stabilize the microtubule network and attenuate the microtubuledestabilizing activity of SCG10. Furthermore, co-expression of BR3 with SCG10 attenuated SCG10-mediated PC12 cell neurite outgrowth induced by NGF. These results identify a novel connection between a neuron-specific BRI protein and the cytoskeletal network, suggesting possible roles of BR3 in the process of neuronal differentiation. [BMB reports 2008; 41(4): 287-293]
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KEYWORD
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Cytoskeletal network, Neuronal differentiation, Neurite outgrowth, Microtubule, PC12
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